|Start -> Run -> (type) SYSEDIT.|
This will bring up the old 16bit INI files kind of the old school registry. Look for WIN.INI. Bring that window to the front by clicking on it. (You have to know a little about old 16bit program manager to navigate) Once it is the active Window scroll down the list until you find either "LOAD=" or "RUN=". Remove the line, save and reboot.
By default you should not have one. One way viruses load unless you truely are running an old 16bit app that automatically starts on boot.