|Well, I don't see any column letters so I guess I'll just have to assume that Columns B and C are the ones with the numbers.|
I also don't see any row numbers, so I guess I'll just have to assume that your data starts in Row 1.
"Calculate the difference" could mean many things, but I don't see any of the "desired output" that I asked for, so I guess I'll just have to assume that by "calculate the difference" you mean "subtract the values".
In D1 enter =B1-C1 or =C1-B1 and drag it down to the bottom of your list.
If your list is contiguous (no gaps) and you use the formula in an adjacent column (e.g. D), you can enter the formula in D1, put your cursor over of the autofill corner and double click. The formula should auto-fill down to bottom of your list.
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