if create a partition on hard disk and copy paste io msdos command com then have a real ms dos in use or not ?
Not. You need to format the partition with the /s switch.
If you're trying to create a MS-DOS partition on a Windows 8 drive (which is what shows in your specs above) then I suggest you stop and rethink. It could be catastrophic to the existing OS. It's much easier and safer to use a virtual-machine. "Channeling the spirit of jboy..."
There's many variables you haven't mentioned--drive size and type, dos version, whether it's a separate drive or a partition on a drive already being used. But in general, as already mentioned, you partition, usually with Fdisk, then format using the /s switch or run the Sys command afterward. Io.sys and Msdos.sys are installed in a particular place on the hard drive. The /s and Sys command put them in the correct place. Copying and pasting those files usually results in a non-booting drive.