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I have a program that I have always used on my computer and all of a sudden when I try to get into the program it says 16 bit MS-DOS Subsystem C:\WINDOWS\System32\autoexec.nt The System file is not suitable for running MS-DOS and Microsoft Windows applications. I have tried uninstalling the program and reinstalled but I still get the 16 bit error message. Any suggestions?

Win XP doesn't use DOS like Win 98. If it's an old DOS based program, it won't run on WinXP.
When all is said and done
More is said than done..

I've had a different experience, and my program ran wonderfully until last week: My wife has a program called, "Managing your money" (Vol 12) a DOS based program from Andrew Tobias. She has been running it these past 2 years under our Windows XP Home software on a HP computer, and it's worked perfectly. Last week when she tried to run the program (and we've not changed anything) she received this message, "C:\Windows\System32\Autoexec.NT: The system file is not suitable for running MS-DOS and Microsoft Windows application." We really haven't done anything, but all of a sudden we're receiving this mesage. An ideas would sure be appriciated, my wife loves this program! Thanks, Rob Burdsal

I'm really hoping someone can help. I am having the exact same problem. I have not updated or changed anything, just like you. It's been working just fine on the computer with XP for 2 years now and last week I started getting that error message. I really don't want to have to do a quick restore and I am hoping someone out there can help me...

If you have a copy of autoexec.nt in windows\repair, copy and paste it into windows\system32.Some adware/spyware will delete the autoexec.nt, so you may want to run a few scans.

I was just told by the people that supplied me with the program, that I am having trouble with, that if you do the step that was suggested above and the next time you reboot your computer you still have the same problem then you have a virus. They said this virus deletes the autoexe.nt file at shut down. They have contacted Microsoft and they said they are aware of it but have no "fix" for it yet. Anyone else heard of this?

I have same problem. I tried to move it from repair to system32. Now it's gone altogether.
I have downloaded ServicePack 2 but only have CD-Rom with ServicePack 1. Can I use it to reinstall file??

I had the same error with windows xp pro "16bit MS-DOS Subsystem - C:\windwows\system32\autoexec.nt - The system file is not suitable for running MSDOS and MS windows applications" I received the error when attempting install Norton AV. This is after running both SPYBOT SD and AdWARE SE Personal. What I did to resolve it was copying "autoexec.nt" from c:\windows\repair folder to c:\windows\system32 folder. Here's how, I did a search on autoexec.nt on my C drive and noticed that I only had one in C:\windows\repair, so I right click on it and selected "copy", then opened "My computer", "windows" folder, "system32" folder, and right click inside the folder and selected "paste". The rest is history. Try it and good luck. Thanks for all your postings and it did help me figure out this problem.
8675309

There was a laptop in the office with this problem yesterday. A tech who worked on it remotely said he fixed it by copying AUTOEXEC.NT from his machine over to the laptop, and setting the file to Read Only. Then scanned with Spybot S&D, Ad-Aware, and TrendMicro's Housecall. The laptop is now error free.

Try this page for info - it seems that this 'problem' has become very prevalent and is probably caused by a trojan
http://windowsxp.mvps.org/16bit.htm
Regards
Shaun

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